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    ATCC human hprec atcc cat
    Human Hprec Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hprec+atcc+cat/Primary+Prostate+Epithelial+Cells%3B+Normal%2C+Human/pm39742810-298-92-94
    Average 99 stars, based on 540 article reviews
    human hprec atcc cat - by Bioz Stars, 2026-10
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    Phospho-proteomics:

    Article Title: The human zinc-binding cysteine proteome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NuPAGE LDS Sample Buffer (4x) Thermo Fisher Scientific Cat#NP0007 NuPAGE MOPS SDS Running Buffer (20x) Thermo Fisher Scientific Cat#NP0001-02 PBS (Phosphate buffered saline) Corning Cat#21-040-CV Penicillin Streptomycin Solution Corning Cat#30-002-CI pMD2.G AddGene Cat#12259 PolyFect Transfection Reagent Qiagen Cat#301105 Polybrene Santa Cruz Biotechnology Cat#sc-134200, CAS#28728-55-4 Potassium Cyanide Sigma-Aldrich Cat#60178; CAS#151-50-8 Prostate Epithelial Cell Growth Kit ATCC Cat#PCS-440-040 Prostate Epithelial Cell Basal Medium ATCC Cat#PCS-440-030 psPAX2 AddGene Cat#12260 Puromycin Gibco Cat#A111138-03 Pyrithione (2-Mercaptopyridine N-oxide) Sigma-Aldrich Cat#188549; CAS#1121-31-9 RIPA buffer Sigma-Aldrich Cat#R0278 Rotenone Sigma-Aldrich Cat#R8875; CAS#83-79-4 TBST (Tris Buffered Saline-Tween) (20x) Boston BioProducts Cat#IBB-180X TCEP solution (Bond Breaker ) Thermo Fisher Scientific Cat#77720 TFA (Trifluoroacetic acid LC-MS grade) Thermo Fisher Scientific Cat#A116-50; CAS#76-05-1 TMTpro reagents Thermo Fisher Scientific Cat# A44520 (16-plex) Cat# A52045 (18-plex) TPEN (N,N,N0,N0-tetrakis(2-pyridinylmethyl)1,2-ethanediamine) Sigma-Aldrich Cat#P4413; CAS#16858-02-9 Triethanolamine Sigma-Aldrich Cat#90279; CAS#102-71-6 Tris 1 M solution pH 7.5 TEKNova Cat# T1075 Sequencing grade modified Trypsin Promega Cat# V5113 Trypsin EDTA (0.25%) solution Gibco Cat#25200-056 Trypsin-EDTA for Primary Cells ATCC Cat# PCS-999-003 Sodium dodecyl sulfate Sigma-Aldrich Cat#L3771; CAS#151-21-3 Strep-Tactin Sepharose resin Iba Lifesciences Cat# 2-1201-002 Succinimidyl iodoacetate (SIA) Combi-Blocks Cat#QC4655; CAS#39028-27-8 5-Sulfosalicylic acid dihydrate Sigma-Aldrich Cat#H247006; CAS#5965-83-3 Zinc Pyrithione Sigma-Aldrich Cat#PHR1401; CAS#13463-41-7 Zinc Chloride Sigma-Aldrich Cat#Z0152; CAS#7646-85-7 Zinc Siacylate trihydrate Spectrum Chemical Cat#Z1042; CAS#16283-36-6 Zinc Sulfate Sigma-Aldrich Cat#307491; CAS#7446-19-7 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat#23225 Pierce Micro BCA Protein Assay Kit Thermo Fisher Scientific Cat#23235 (Continued on next page) ll OPEN ACCESS e3 Cell 188, 1–19.e1–e15, February 6, 2025 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Fisher Scientific Cat#A32992 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Fisher Scientific Cat#84868 Cell Titer-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Protein Labeling Kit RED-NHS 2nd Generation NanoTemper Cat#MO-L011 Deposited data ZnCPT Cysteine Proteomic data This study https://www.proteomexchange.org PRIDE: PXD057034 Experimental Models: Cell lines A549 cells ATCC Cat#CCL-185 HCT116 cells ATCC Cat#CCL-247 SK-MES-1 (SKMES1) cells ATCC Cat#HTB-58 Lenti-X 293T cells Takara Bio USA Cat#632180 DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donors, Mixed Gender Lonza Cat#HUCS10P Primary Prostate Epithelial Cells; Normal, Human (HPrEC) ATCC Cat#PCS-440-010 Experimental Models: Organisms/strains Nude BALB/c mice (homozygous; female) Charles River Laboratories Cat#194 C57BL/6 mice Charles River Laboratories Cat#027 Software and algorithms GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ In-house mass spectrometry data analysis software Huttlin et al.104 N/A MEME Suite Bailey et al.105 https://meme-suite.org/meme/ MO Affinity Analysis Software NanoTemper N/A NanoAnalyze software TA Instruments N/A Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator.html PyMol version 2.3.1 / 2.5 Schroedinger https://pymol.org/2/ Python 3.7 Python Software Foundation https://www.python.org R version 4.2.1 R Project https://www.r-project.org R Studio version 2022.07.2 Posti, Joseph J. Allaire https://posit.co/downloads/ Thermo Xcalibur v1.4 Thermo Fisher Scientific N/A Other Affinity TIC TA Instruments N/A Amicon Ultra Centrifugal Filter 30K MWCO 4 ml Millipore Cat#UFC8030 BL21(DE3) competent cells Thermo Fisher Scientific Cat#EC0114 ClarioSTAR Plus BMG Labtech N/A Capillaries (Monolith) NanoTemper Cat#MO-K022 EASY-nLC 1200 System Thermo Fisher Scientific Cat#LC140 EASY-Spray C18 HPLC column Thermo Fisher Scientific Cat#ES902 HiLoad 16/600 Superdex 200 pg cytiva Cat#28989335 iBlot 2 dry blotting system Thermo Fisher Scientific N/A iBlot 2 PVDF Mini Stacks Thermo Fisher Scientific Cat#IB24002 Monolith NT.115 NanoTemper N/A NuPAGETM 4 to 12 % Bis-Tris gels Thermo Fisher Scientific Cat#NP0335BOX Odyssey DLx LiCor N/A Orbitrap Eclipse Tribrid Mass Spectrometer Thermo Fisher Scientific Cat#FSN04-10000 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e15, February 6, 2025 e4 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource ..

    Peptide Fractionation:

    Article Title: The human zinc-binding cysteine proteome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NuPAGE LDS Sample Buffer (4x) Thermo Fisher Scientific Cat#NP0007 NuPAGE MOPS SDS Running Buffer (20x) Thermo Fisher Scientific Cat#NP0001-02 PBS (Phosphate buffered saline) Corning Cat#21-040-CV Penicillin Streptomycin Solution Corning Cat#30-002-CI pMD2.G AddGene Cat#12259 PolyFect Transfection Reagent Qiagen Cat#301105 Polybrene Santa Cruz Biotechnology Cat#sc-134200, CAS#28728-55-4 Potassium Cyanide Sigma-Aldrich Cat#60178; CAS#151-50-8 Prostate Epithelial Cell Growth Kit ATCC Cat#PCS-440-040 Prostate Epithelial Cell Basal Medium ATCC Cat#PCS-440-030 psPAX2 AddGene Cat#12260 Puromycin Gibco Cat#A111138-03 Pyrithione (2-Mercaptopyridine N-oxide) Sigma-Aldrich Cat#188549; CAS#1121-31-9 RIPA buffer Sigma-Aldrich Cat#R0278 Rotenone Sigma-Aldrich Cat#R8875; CAS#83-79-4 TBST (Tris Buffered Saline-Tween) (20x) Boston BioProducts Cat#IBB-180X TCEP solution (Bond Breaker ) Thermo Fisher Scientific Cat#77720 TFA (Trifluoroacetic acid LC-MS grade) Thermo Fisher Scientific Cat#A116-50; CAS#76-05-1 TMTpro reagents Thermo Fisher Scientific Cat# A44520 (16-plex) Cat# A52045 (18-plex) TPEN (N,N,N0,N0-tetrakis(2-pyridinylmethyl)1,2-ethanediamine) Sigma-Aldrich Cat#P4413; CAS#16858-02-9 Triethanolamine Sigma-Aldrich Cat#90279; CAS#102-71-6 Tris 1 M solution pH 7.5 TEKNova Cat# T1075 Sequencing grade modified Trypsin Promega Cat# V5113 Trypsin EDTA (0.25%) solution Gibco Cat#25200-056 Trypsin-EDTA for Primary Cells ATCC Cat# PCS-999-003 Sodium dodecyl sulfate Sigma-Aldrich Cat#L3771; CAS#151-21-3 Strep-Tactin Sepharose resin Iba Lifesciences Cat# 2-1201-002 Succinimidyl iodoacetate (SIA) Combi-Blocks Cat#QC4655; CAS#39028-27-8 5-Sulfosalicylic acid dihydrate Sigma-Aldrich Cat#H247006; CAS#5965-83-3 Zinc Pyrithione Sigma-Aldrich Cat#PHR1401; CAS#13463-41-7 Zinc Chloride Sigma-Aldrich Cat#Z0152; CAS#7646-85-7 Zinc Siacylate trihydrate Spectrum Chemical Cat#Z1042; CAS#16283-36-6 Zinc Sulfate Sigma-Aldrich Cat#307491; CAS#7446-19-7 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat#23225 Pierce Micro BCA Protein Assay Kit Thermo Fisher Scientific Cat#23235 (Continued on next page) ll OPEN ACCESS e3 Cell 188, 1–19.e1–e15, February 6, 2025 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Fisher Scientific Cat#A32992 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Fisher Scientific Cat#84868 Cell Titer-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Protein Labeling Kit RED-NHS 2nd Generation NanoTemper Cat#MO-L011 Deposited data ZnCPT Cysteine Proteomic data This study https://www.proteomexchange.org PRIDE: PXD057034 Experimental Models: Cell lines A549 cells ATCC Cat#CCL-185 HCT116 cells ATCC Cat#CCL-247 SK-MES-1 (SKMES1) cells ATCC Cat#HTB-58 Lenti-X 293T cells Takara Bio USA Cat#632180 DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donors, Mixed Gender Lonza Cat#HUCS10P Primary Prostate Epithelial Cells; Normal, Human (HPrEC) ATCC Cat#PCS-440-010 Experimental Models: Organisms/strains Nude BALB/c mice (homozygous; female) Charles River Laboratories Cat#194 C57BL/6 mice Charles River Laboratories Cat#027 Software and algorithms GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ In-house mass spectrometry data analysis software Huttlin et al.104 N/A MEME Suite Bailey et al.105 https://meme-suite.org/meme/ MO Affinity Analysis Software NanoTemper N/A NanoAnalyze software TA Instruments N/A Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator.html PyMol version 2.3.1 / 2.5 Schroedinger https://pymol.org/2/ Python 3.7 Python Software Foundation https://www.python.org R version 4.2.1 R Project https://www.r-project.org R Studio version 2022.07.2 Posti, Joseph J. Allaire https://posit.co/downloads/ Thermo Xcalibur v1.4 Thermo Fisher Scientific N/A Other Affinity TIC TA Instruments N/A Amicon Ultra Centrifugal Filter 30K MWCO 4 ml Millipore Cat#UFC8030 BL21(DE3) competent cells Thermo Fisher Scientific Cat#EC0114 ClarioSTAR Plus BMG Labtech N/A Capillaries (Monolith) NanoTemper Cat#MO-K022 EASY-nLC 1200 System Thermo Fisher Scientific Cat#LC140 EASY-Spray C18 HPLC column Thermo Fisher Scientific Cat#ES902 HiLoad 16/600 Superdex 200 pg cytiva Cat#28989335 iBlot 2 dry blotting system Thermo Fisher Scientific N/A iBlot 2 PVDF Mini Stacks Thermo Fisher Scientific Cat#IB24002 Monolith NT.115 NanoTemper N/A NuPAGETM 4 to 12 % Bis-Tris gels Thermo Fisher Scientific Cat#NP0335BOX Odyssey DLx LiCor N/A Orbitrap Eclipse Tribrid Mass Spectrometer Thermo Fisher Scientific Cat#FSN04-10000 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e15, February 6, 2025 e4 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource ..

    Cell Viability Assay:

    Article Title: The human zinc-binding cysteine proteome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NuPAGE LDS Sample Buffer (4x) Thermo Fisher Scientific Cat#NP0007 NuPAGE MOPS SDS Running Buffer (20x) Thermo Fisher Scientific Cat#NP0001-02 PBS (Phosphate buffered saline) Corning Cat#21-040-CV Penicillin Streptomycin Solution Corning Cat#30-002-CI pMD2.G AddGene Cat#12259 PolyFect Transfection Reagent Qiagen Cat#301105 Polybrene Santa Cruz Biotechnology Cat#sc-134200, CAS#28728-55-4 Potassium Cyanide Sigma-Aldrich Cat#60178; CAS#151-50-8 Prostate Epithelial Cell Growth Kit ATCC Cat#PCS-440-040 Prostate Epithelial Cell Basal Medium ATCC Cat#PCS-440-030 psPAX2 AddGene Cat#12260 Puromycin Gibco Cat#A111138-03 Pyrithione (2-Mercaptopyridine N-oxide) Sigma-Aldrich Cat#188549; CAS#1121-31-9 RIPA buffer Sigma-Aldrich Cat#R0278 Rotenone Sigma-Aldrich Cat#R8875; CAS#83-79-4 TBST (Tris Buffered Saline-Tween) (20x) Boston BioProducts Cat#IBB-180X TCEP solution (Bond Breaker ) Thermo Fisher Scientific Cat#77720 TFA (Trifluoroacetic acid LC-MS grade) Thermo Fisher Scientific Cat#A116-50; CAS#76-05-1 TMTpro reagents Thermo Fisher Scientific Cat# A44520 (16-plex) Cat# A52045 (18-plex) TPEN (N,N,N0,N0-tetrakis(2-pyridinylmethyl)1,2-ethanediamine) Sigma-Aldrich Cat#P4413; CAS#16858-02-9 Triethanolamine Sigma-Aldrich Cat#90279; CAS#102-71-6 Tris 1 M solution pH 7.5 TEKNova Cat# T1075 Sequencing grade modified Trypsin Promega Cat# V5113 Trypsin EDTA (0.25%) solution Gibco Cat#25200-056 Trypsin-EDTA for Primary Cells ATCC Cat# PCS-999-003 Sodium dodecyl sulfate Sigma-Aldrich Cat#L3771; CAS#151-21-3 Strep-Tactin Sepharose resin Iba Lifesciences Cat# 2-1201-002 Succinimidyl iodoacetate (SIA) Combi-Blocks Cat#QC4655; CAS#39028-27-8 5-Sulfosalicylic acid dihydrate Sigma-Aldrich Cat#H247006; CAS#5965-83-3 Zinc Pyrithione Sigma-Aldrich Cat#PHR1401; CAS#13463-41-7 Zinc Chloride Sigma-Aldrich Cat#Z0152; CAS#7646-85-7 Zinc Siacylate trihydrate Spectrum Chemical Cat#Z1042; CAS#16283-36-6 Zinc Sulfate Sigma-Aldrich Cat#307491; CAS#7446-19-7 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat#23225 Pierce Micro BCA Protein Assay Kit Thermo Fisher Scientific Cat#23235 (Continued on next page) ll OPEN ACCESS e3 Cell 188, 1–19.e1–e15, February 6, 2025 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Fisher Scientific Cat#A32992 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Fisher Scientific Cat#84868 Cell Titer-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Protein Labeling Kit RED-NHS 2nd Generation NanoTemper Cat#MO-L011 Deposited data ZnCPT Cysteine Proteomic data This study https://www.proteomexchange.org PRIDE: PXD057034 Experimental Models: Cell lines A549 cells ATCC Cat#CCL-185 HCT116 cells ATCC Cat#CCL-247 SK-MES-1 (SKMES1) cells ATCC Cat#HTB-58 Lenti-X 293T cells Takara Bio USA Cat#632180 DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donors, Mixed Gender Lonza Cat#HUCS10P Primary Prostate Epithelial Cells; Normal, Human (HPrEC) ATCC Cat#PCS-440-010 Experimental Models: Organisms/strains Nude BALB/c mice (homozygous; female) Charles River Laboratories Cat#194 C57BL/6 mice Charles River Laboratories Cat#027 Software and algorithms GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ In-house mass spectrometry data analysis software Huttlin et al.104 N/A MEME Suite Bailey et al.105 https://meme-suite.org/meme/ MO Affinity Analysis Software NanoTemper N/A NanoAnalyze software TA Instruments N/A Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator.html PyMol version 2.3.1 / 2.5 Schroedinger https://pymol.org/2/ Python 3.7 Python Software Foundation https://www.python.org R version 4.2.1 R Project https://www.r-project.org R Studio version 2022.07.2 Posti, Joseph J. Allaire https://posit.co/downloads/ Thermo Xcalibur v1.4 Thermo Fisher Scientific N/A Other Affinity TIC TA Instruments N/A Amicon Ultra Centrifugal Filter 30K MWCO 4 ml Millipore Cat#UFC8030 BL21(DE3) competent cells Thermo Fisher Scientific Cat#EC0114 ClarioSTAR Plus BMG Labtech N/A Capillaries (Monolith) NanoTemper Cat#MO-K022 EASY-nLC 1200 System Thermo Fisher Scientific Cat#LC140 EASY-Spray C18 HPLC column Thermo Fisher Scientific Cat#ES902 HiLoad 16/600 Superdex 200 pg cytiva Cat#28989335 iBlot 2 dry blotting system Thermo Fisher Scientific N/A iBlot 2 PVDF Mini Stacks Thermo Fisher Scientific Cat#IB24002 Monolith NT.115 NanoTemper N/A NuPAGETM 4 to 12 % Bis-Tris gels Thermo Fisher Scientific Cat#NP0335BOX Odyssey DLx LiCor N/A Orbitrap Eclipse Tribrid Mass Spectrometer Thermo Fisher Scientific Cat#FSN04-10000 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e15, February 6, 2025 e4 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource ..

    Labeling:

    Article Title: The human zinc-binding cysteine proteome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NuPAGE LDS Sample Buffer (4x) Thermo Fisher Scientific Cat#NP0007 NuPAGE MOPS SDS Running Buffer (20x) Thermo Fisher Scientific Cat#NP0001-02 PBS (Phosphate buffered saline) Corning Cat#21-040-CV Penicillin Streptomycin Solution Corning Cat#30-002-CI pMD2.G AddGene Cat#12259 PolyFect Transfection Reagent Qiagen Cat#301105 Polybrene Santa Cruz Biotechnology Cat#sc-134200, CAS#28728-55-4 Potassium Cyanide Sigma-Aldrich Cat#60178; CAS#151-50-8 Prostate Epithelial Cell Growth Kit ATCC Cat#PCS-440-040 Prostate Epithelial Cell Basal Medium ATCC Cat#PCS-440-030 psPAX2 AddGene Cat#12260 Puromycin Gibco Cat#A111138-03 Pyrithione (2-Mercaptopyridine N-oxide) Sigma-Aldrich Cat#188549; CAS#1121-31-9 RIPA buffer Sigma-Aldrich Cat#R0278 Rotenone Sigma-Aldrich Cat#R8875; CAS#83-79-4 TBST (Tris Buffered Saline-Tween) (20x) Boston BioProducts Cat#IBB-180X TCEP solution (Bond Breaker ) Thermo Fisher Scientific Cat#77720 TFA (Trifluoroacetic acid LC-MS grade) Thermo Fisher Scientific Cat#A116-50; CAS#76-05-1 TMTpro reagents Thermo Fisher Scientific Cat# A44520 (16-plex) Cat# A52045 (18-plex) TPEN (N,N,N0,N0-tetrakis(2-pyridinylmethyl)1,2-ethanediamine) Sigma-Aldrich Cat#P4413; CAS#16858-02-9 Triethanolamine Sigma-Aldrich Cat#90279; CAS#102-71-6 Tris 1 M solution pH 7.5 TEKNova Cat# T1075 Sequencing grade modified Trypsin Promega Cat# V5113 Trypsin EDTA (0.25%) solution Gibco Cat#25200-056 Trypsin-EDTA for Primary Cells ATCC Cat# PCS-999-003 Sodium dodecyl sulfate Sigma-Aldrich Cat#L3771; CAS#151-21-3 Strep-Tactin Sepharose resin Iba Lifesciences Cat# 2-1201-002 Succinimidyl iodoacetate (SIA) Combi-Blocks Cat#QC4655; CAS#39028-27-8 5-Sulfosalicylic acid dihydrate Sigma-Aldrich Cat#H247006; CAS#5965-83-3 Zinc Pyrithione Sigma-Aldrich Cat#PHR1401; CAS#13463-41-7 Zinc Chloride Sigma-Aldrich Cat#Z0152; CAS#7646-85-7 Zinc Siacylate trihydrate Spectrum Chemical Cat#Z1042; CAS#16283-36-6 Zinc Sulfate Sigma-Aldrich Cat#307491; CAS#7446-19-7 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat#23225 Pierce Micro BCA Protein Assay Kit Thermo Fisher Scientific Cat#23235 (Continued on next page) ll OPEN ACCESS e3 Cell 188, 1–19.e1–e15, February 6, 2025 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Fisher Scientific Cat#A32992 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Fisher Scientific Cat#84868 Cell Titer-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Protein Labeling Kit RED-NHS 2nd Generation NanoTemper Cat#MO-L011 Deposited data ZnCPT Cysteine Proteomic data This study https://www.proteomexchange.org PRIDE: PXD057034 Experimental Models: Cell lines A549 cells ATCC Cat#CCL-185 HCT116 cells ATCC Cat#CCL-247 SK-MES-1 (SKMES1) cells ATCC Cat#HTB-58 Lenti-X 293T cells Takara Bio USA Cat#632180 DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donors, Mixed Gender Lonza Cat#HUCS10P Primary Prostate Epithelial Cells; Normal, Human (HPrEC) ATCC Cat#PCS-440-010 Experimental Models: Organisms/strains Nude BALB/c mice (homozygous; female) Charles River Laboratories Cat#194 C57BL/6 mice Charles River Laboratories Cat#027 Software and algorithms GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ In-house mass spectrometry data analysis software Huttlin et al.104 N/A MEME Suite Bailey et al.105 https://meme-suite.org/meme/ MO Affinity Analysis Software NanoTemper N/A NanoAnalyze software TA Instruments N/A Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator.html PyMol version 2.3.1 / 2.5 Schroedinger https://pymol.org/2/ Python 3.7 Python Software Foundation https://www.python.org R version 4.2.1 R Project https://www.r-project.org R Studio version 2022.07.2 Posti, Joseph J. Allaire https://posit.co/downloads/ Thermo Xcalibur v1.4 Thermo Fisher Scientific N/A Other Affinity TIC TA Instruments N/A Amicon Ultra Centrifugal Filter 30K MWCO 4 ml Millipore Cat#UFC8030 BL21(DE3) competent cells Thermo Fisher Scientific Cat#EC0114 ClarioSTAR Plus BMG Labtech N/A Capillaries (Monolith) NanoTemper Cat#MO-K022 EASY-nLC 1200 System Thermo Fisher Scientific Cat#LC140 EASY-Spray C18 HPLC column Thermo Fisher Scientific Cat#ES902 HiLoad 16/600 Superdex 200 pg cytiva Cat#28989335 iBlot 2 dry blotting system Thermo Fisher Scientific N/A iBlot 2 PVDF Mini Stacks Thermo Fisher Scientific Cat#IB24002 Monolith NT.115 NanoTemper N/A NuPAGETM 4 to 12 % Bis-Tris gels Thermo Fisher Scientific Cat#NP0335BOX Odyssey DLx LiCor N/A Orbitrap Eclipse Tribrid Mass Spectrometer Thermo Fisher Scientific Cat#FSN04-10000 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e15, February 6, 2025 e4 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource ..

    Software:

    Article Title: The human zinc-binding cysteine proteome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NuPAGE LDS Sample Buffer (4x) Thermo Fisher Scientific Cat#NP0007 NuPAGE MOPS SDS Running Buffer (20x) Thermo Fisher Scientific Cat#NP0001-02 PBS (Phosphate buffered saline) Corning Cat#21-040-CV Penicillin Streptomycin Solution Corning Cat#30-002-CI pMD2.G AddGene Cat#12259 PolyFect Transfection Reagent Qiagen Cat#301105 Polybrene Santa Cruz Biotechnology Cat#sc-134200, CAS#28728-55-4 Potassium Cyanide Sigma-Aldrich Cat#60178; CAS#151-50-8 Prostate Epithelial Cell Growth Kit ATCC Cat#PCS-440-040 Prostate Epithelial Cell Basal Medium ATCC Cat#PCS-440-030 psPAX2 AddGene Cat#12260 Puromycin Gibco Cat#A111138-03 Pyrithione (2-Mercaptopyridine N-oxide) Sigma-Aldrich Cat#188549; CAS#1121-31-9 RIPA buffer Sigma-Aldrich Cat#R0278 Rotenone Sigma-Aldrich Cat#R8875; CAS#83-79-4 TBST (Tris Buffered Saline-Tween) (20x) Boston BioProducts Cat#IBB-180X TCEP solution (Bond Breaker ) Thermo Fisher Scientific Cat#77720 TFA (Trifluoroacetic acid LC-MS grade) Thermo Fisher Scientific Cat#A116-50; CAS#76-05-1 TMTpro reagents Thermo Fisher Scientific Cat# A44520 (16-plex) Cat# A52045 (18-plex) TPEN (N,N,N0,N0-tetrakis(2-pyridinylmethyl)1,2-ethanediamine) Sigma-Aldrich Cat#P4413; CAS#16858-02-9 Triethanolamine Sigma-Aldrich Cat#90279; CAS#102-71-6 Tris 1 M solution pH 7.5 TEKNova Cat# T1075 Sequencing grade modified Trypsin Promega Cat# V5113 Trypsin EDTA (0.25%) solution Gibco Cat#25200-056 Trypsin-EDTA for Primary Cells ATCC Cat# PCS-999-003 Sodium dodecyl sulfate Sigma-Aldrich Cat#L3771; CAS#151-21-3 Strep-Tactin Sepharose resin Iba Lifesciences Cat# 2-1201-002 Succinimidyl iodoacetate (SIA) Combi-Blocks Cat#QC4655; CAS#39028-27-8 5-Sulfosalicylic acid dihydrate Sigma-Aldrich Cat#H247006; CAS#5965-83-3 Zinc Pyrithione Sigma-Aldrich Cat#PHR1401; CAS#13463-41-7 Zinc Chloride Sigma-Aldrich Cat#Z0152; CAS#7646-85-7 Zinc Siacylate trihydrate Spectrum Chemical Cat#Z1042; CAS#16283-36-6 Zinc Sulfate Sigma-Aldrich Cat#307491; CAS#7446-19-7 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat#23225 Pierce Micro BCA Protein Assay Kit Thermo Fisher Scientific Cat#23235 (Continued on next page) ll OPEN ACCESS e3 Cell 188, 1–19.e1–e15, February 6, 2025 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Fisher Scientific Cat#A32992 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Fisher Scientific Cat#84868 Cell Titer-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Protein Labeling Kit RED-NHS 2nd Generation NanoTemper Cat#MO-L011 Deposited data ZnCPT Cysteine Proteomic data This study https://www.proteomexchange.org PRIDE: PXD057034 Experimental Models: Cell lines A549 cells ATCC Cat#CCL-185 HCT116 cells ATCC Cat#CCL-247 SK-MES-1 (SKMES1) cells ATCC Cat#HTB-58 Lenti-X 293T cells Takara Bio USA Cat#632180 DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donors, Mixed Gender Lonza Cat#HUCS10P Primary Prostate Epithelial Cells; Normal, Human (HPrEC) ATCC Cat#PCS-440-010 Experimental Models: Organisms/strains Nude BALB/c mice (homozygous; female) Charles River Laboratories Cat#194 C57BL/6 mice Charles River Laboratories Cat#027 Software and algorithms GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ In-house mass spectrometry data analysis software Huttlin et al.104 N/A MEME Suite Bailey et al.105 https://meme-suite.org/meme/ MO Affinity Analysis Software NanoTemper N/A NanoAnalyze software TA Instruments N/A Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator.html PyMol version 2.3.1 / 2.5 Schroedinger https://pymol.org/2/ Python 3.7 Python Software Foundation https://www.python.org R version 4.2.1 R Project https://www.r-project.org R Studio version 2022.07.2 Posti, Joseph J. Allaire https://posit.co/downloads/ Thermo Xcalibur v1.4 Thermo Fisher Scientific N/A Other Affinity TIC TA Instruments N/A Amicon Ultra Centrifugal Filter 30K MWCO 4 ml Millipore Cat#UFC8030 BL21(DE3) competent cells Thermo Fisher Scientific Cat#EC0114 ClarioSTAR Plus BMG Labtech N/A Capillaries (Monolith) NanoTemper Cat#MO-K022 EASY-nLC 1200 System Thermo Fisher Scientific Cat#LC140 EASY-Spray C18 HPLC column Thermo Fisher Scientific Cat#ES902 HiLoad 16/600 Superdex 200 pg cytiva Cat#28989335 iBlot 2 dry blotting system Thermo Fisher Scientific N/A iBlot 2 PVDF Mini Stacks Thermo Fisher Scientific Cat#IB24002 Monolith NT.115 NanoTemper N/A NuPAGETM 4 to 12 % Bis-Tris gels Thermo Fisher Scientific Cat#NP0335BOX Odyssey DLx LiCor N/A Orbitrap Eclipse Tribrid Mass Spectrometer Thermo Fisher Scientific Cat#FSN04-10000 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e15, February 6, 2025 e4 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource ..

    Mass Spectrometry:

    Article Title: The human zinc-binding cysteine proteome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NuPAGE LDS Sample Buffer (4x) Thermo Fisher Scientific Cat#NP0007 NuPAGE MOPS SDS Running Buffer (20x) Thermo Fisher Scientific Cat#NP0001-02 PBS (Phosphate buffered saline) Corning Cat#21-040-CV Penicillin Streptomycin Solution Corning Cat#30-002-CI pMD2.G AddGene Cat#12259 PolyFect Transfection Reagent Qiagen Cat#301105 Polybrene Santa Cruz Biotechnology Cat#sc-134200, CAS#28728-55-4 Potassium Cyanide Sigma-Aldrich Cat#60178; CAS#151-50-8 Prostate Epithelial Cell Growth Kit ATCC Cat#PCS-440-040 Prostate Epithelial Cell Basal Medium ATCC Cat#PCS-440-030 psPAX2 AddGene Cat#12260 Puromycin Gibco Cat#A111138-03 Pyrithione (2-Mercaptopyridine N-oxide) Sigma-Aldrich Cat#188549; CAS#1121-31-9 RIPA buffer Sigma-Aldrich Cat#R0278 Rotenone Sigma-Aldrich Cat#R8875; CAS#83-79-4 TBST (Tris Buffered Saline-Tween) (20x) Boston BioProducts Cat#IBB-180X TCEP solution (Bond Breaker ) Thermo Fisher Scientific Cat#77720 TFA (Trifluoroacetic acid LC-MS grade) Thermo Fisher Scientific Cat#A116-50; CAS#76-05-1 TMTpro reagents Thermo Fisher Scientific Cat# A44520 (16-plex) Cat# A52045 (18-plex) TPEN (N,N,N0,N0-tetrakis(2-pyridinylmethyl)1,2-ethanediamine) Sigma-Aldrich Cat#P4413; CAS#16858-02-9 Triethanolamine Sigma-Aldrich Cat#90279; CAS#102-71-6 Tris 1 M solution pH 7.5 TEKNova Cat# T1075 Sequencing grade modified Trypsin Promega Cat# V5113 Trypsin EDTA (0.25%) solution Gibco Cat#25200-056 Trypsin-EDTA for Primary Cells ATCC Cat# PCS-999-003 Sodium dodecyl sulfate Sigma-Aldrich Cat#L3771; CAS#151-21-3 Strep-Tactin Sepharose resin Iba Lifesciences Cat# 2-1201-002 Succinimidyl iodoacetate (SIA) Combi-Blocks Cat#QC4655; CAS#39028-27-8 5-Sulfosalicylic acid dihydrate Sigma-Aldrich Cat#H247006; CAS#5965-83-3 Zinc Pyrithione Sigma-Aldrich Cat#PHR1401; CAS#13463-41-7 Zinc Chloride Sigma-Aldrich Cat#Z0152; CAS#7646-85-7 Zinc Siacylate trihydrate Spectrum Chemical Cat#Z1042; CAS#16283-36-6 Zinc Sulfate Sigma-Aldrich Cat#307491; CAS#7446-19-7 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat#23225 Pierce Micro BCA Protein Assay Kit Thermo Fisher Scientific Cat#23235 (Continued on next page) ll OPEN ACCESS e3 Cell 188, 1–19.e1–e15, February 6, 2025 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Fisher Scientific Cat#A32992 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Fisher Scientific Cat#84868 Cell Titer-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Protein Labeling Kit RED-NHS 2nd Generation NanoTemper Cat#MO-L011 Deposited data ZnCPT Cysteine Proteomic data This study https://www.proteomexchange.org PRIDE: PXD057034 Experimental Models: Cell lines A549 cells ATCC Cat#CCL-185 HCT116 cells ATCC Cat#CCL-247 SK-MES-1 (SKMES1) cells ATCC Cat#HTB-58 Lenti-X 293T cells Takara Bio USA Cat#632180 DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donors, Mixed Gender Lonza Cat#HUCS10P Primary Prostate Epithelial Cells; Normal, Human (HPrEC) ATCC Cat#PCS-440-010 Experimental Models: Organisms/strains Nude BALB/c mice (homozygous; female) Charles River Laboratories Cat#194 C57BL/6 mice Charles River Laboratories Cat#027 Software and algorithms GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ In-house mass spectrometry data analysis software Huttlin et al.104 N/A MEME Suite Bailey et al.105 https://meme-suite.org/meme/ MO Affinity Analysis Software NanoTemper N/A NanoAnalyze software TA Instruments N/A Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator.html PyMol version 2.3.1 / 2.5 Schroedinger https://pymol.org/2/ Python 3.7 Python Software Foundation https://www.python.org R version 4.2.1 R Project https://www.r-project.org R Studio version 2022.07.2 Posti, Joseph J. Allaire https://posit.co/downloads/ Thermo Xcalibur v1.4 Thermo Fisher Scientific N/A Other Affinity TIC TA Instruments N/A Amicon Ultra Centrifugal Filter 30K MWCO 4 ml Millipore Cat#UFC8030 BL21(DE3) competent cells Thermo Fisher Scientific Cat#EC0114 ClarioSTAR Plus BMG Labtech N/A Capillaries (Monolith) NanoTemper Cat#MO-K022 EASY-nLC 1200 System Thermo Fisher Scientific Cat#LC140 EASY-Spray C18 HPLC column Thermo Fisher Scientific Cat#ES902 HiLoad 16/600 Superdex 200 pg cytiva Cat#28989335 iBlot 2 dry blotting system Thermo Fisher Scientific N/A iBlot 2 PVDF Mini Stacks Thermo Fisher Scientific Cat#IB24002 Monolith NT.115 NanoTemper N/A NuPAGETM 4 to 12 % Bis-Tris gels Thermo Fisher Scientific Cat#NP0335BOX Odyssey DLx LiCor N/A Orbitrap Eclipse Tribrid Mass Spectrometer Thermo Fisher Scientific Cat#FSN04-10000 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e15, February 6, 2025 e4 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource ..

    High Performance Liquid Chromatography:

    Article Title: The human zinc-binding cysteine proteome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NuPAGE LDS Sample Buffer (4x) Thermo Fisher Scientific Cat#NP0007 NuPAGE MOPS SDS Running Buffer (20x) Thermo Fisher Scientific Cat#NP0001-02 PBS (Phosphate buffered saline) Corning Cat#21-040-CV Penicillin Streptomycin Solution Corning Cat#30-002-CI pMD2.G AddGene Cat#12259 PolyFect Transfection Reagent Qiagen Cat#301105 Polybrene Santa Cruz Biotechnology Cat#sc-134200, CAS#28728-55-4 Potassium Cyanide Sigma-Aldrich Cat#60178; CAS#151-50-8 Prostate Epithelial Cell Growth Kit ATCC Cat#PCS-440-040 Prostate Epithelial Cell Basal Medium ATCC Cat#PCS-440-030 psPAX2 AddGene Cat#12260 Puromycin Gibco Cat#A111138-03 Pyrithione (2-Mercaptopyridine N-oxide) Sigma-Aldrich Cat#188549; CAS#1121-31-9 RIPA buffer Sigma-Aldrich Cat#R0278 Rotenone Sigma-Aldrich Cat#R8875; CAS#83-79-4 TBST (Tris Buffered Saline-Tween) (20x) Boston BioProducts Cat#IBB-180X TCEP solution (Bond Breaker ) Thermo Fisher Scientific Cat#77720 TFA (Trifluoroacetic acid LC-MS grade) Thermo Fisher Scientific Cat#A116-50; CAS#76-05-1 TMTpro reagents Thermo Fisher Scientific Cat# A44520 (16-plex) Cat# A52045 (18-plex) TPEN (N,N,N0,N0-tetrakis(2-pyridinylmethyl)1,2-ethanediamine) Sigma-Aldrich Cat#P4413; CAS#16858-02-9 Triethanolamine Sigma-Aldrich Cat#90279; CAS#102-71-6 Tris 1 M solution pH 7.5 TEKNova Cat# T1075 Sequencing grade modified Trypsin Promega Cat# V5113 Trypsin EDTA (0.25%) solution Gibco Cat#25200-056 Trypsin-EDTA for Primary Cells ATCC Cat# PCS-999-003 Sodium dodecyl sulfate Sigma-Aldrich Cat#L3771; CAS#151-21-3 Strep-Tactin Sepharose resin Iba Lifesciences Cat# 2-1201-002 Succinimidyl iodoacetate (SIA) Combi-Blocks Cat#QC4655; CAS#39028-27-8 5-Sulfosalicylic acid dihydrate Sigma-Aldrich Cat#H247006; CAS#5965-83-3 Zinc Pyrithione Sigma-Aldrich Cat#PHR1401; CAS#13463-41-7 Zinc Chloride Sigma-Aldrich Cat#Z0152; CAS#7646-85-7 Zinc Siacylate trihydrate Spectrum Chemical Cat#Z1042; CAS#16283-36-6 Zinc Sulfate Sigma-Aldrich Cat#307491; CAS#7446-19-7 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat#23225 Pierce Micro BCA Protein Assay Kit Thermo Fisher Scientific Cat#23235 (Continued on next page) ll OPEN ACCESS e3 Cell 188, 1–19.e1–e15, February 6, 2025 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Fisher Scientific Cat#A32992 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Fisher Scientific Cat#84868 Cell Titer-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Protein Labeling Kit RED-NHS 2nd Generation NanoTemper Cat#MO-L011 Deposited data ZnCPT Cysteine Proteomic data This study https://www.proteomexchange.org PRIDE: PXD057034 Experimental Models: Cell lines A549 cells ATCC Cat#CCL-185 HCT116 cells ATCC Cat#CCL-247 SK-MES-1 (SKMES1) cells ATCC Cat#HTB-58 Lenti-X 293T cells Takara Bio USA Cat#632180 DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donors, Mixed Gender Lonza Cat#HUCS10P Primary Prostate Epithelial Cells; Normal, Human (HPrEC) ATCC Cat#PCS-440-010 Experimental Models: Organisms/strains Nude BALB/c mice (homozygous; female) Charles River Laboratories Cat#194 C57BL/6 mice Charles River Laboratories Cat#027 Software and algorithms GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ In-house mass spectrometry data analysis software Huttlin et al.104 N/A MEME Suite Bailey et al.105 https://meme-suite.org/meme/ MO Affinity Analysis Software NanoTemper N/A NanoAnalyze software TA Instruments N/A Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator.html PyMol version 2.3.1 / 2.5 Schroedinger https://pymol.org/2/ Python 3.7 Python Software Foundation https://www.python.org R version 4.2.1 R Project https://www.r-project.org R Studio version 2022.07.2 Posti, Joseph J. Allaire https://posit.co/downloads/ Thermo Xcalibur v1.4 Thermo Fisher Scientific N/A Other Affinity TIC TA Instruments N/A Amicon Ultra Centrifugal Filter 30K MWCO 4 ml Millipore Cat#UFC8030 BL21(DE3) competent cells Thermo Fisher Scientific Cat#EC0114 ClarioSTAR Plus BMG Labtech N/A Capillaries (Monolith) NanoTemper Cat#MO-K022 EASY-nLC 1200 System Thermo Fisher Scientific Cat#LC140 EASY-Spray C18 HPLC column Thermo Fisher Scientific Cat#ES902 HiLoad 16/600 Superdex 200 pg cytiva Cat#28989335 iBlot 2 dry blotting system Thermo Fisher Scientific N/A iBlot 2 PVDF Mini Stacks Thermo Fisher Scientific Cat#IB24002 Monolith NT.115 NanoTemper N/A NuPAGETM 4 to 12 % Bis-Tris gels Thermo Fisher Scientific Cat#NP0335BOX Odyssey DLx LiCor N/A Orbitrap Eclipse Tribrid Mass Spectrometer Thermo Fisher Scientific Cat#FSN04-10000 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e15, February 6, 2025 e4 Please cite this article in press as: Burger et al., The human zinc-binding cysteine proteome, Cell (2025), https://doi.org/10.1016/ j.cell.2024.11.025 Resource ..



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